Fig 3. Levels of H3.3BK27R mutant histones determine the impact on development.

(A) Confocal images of salivary glands from wild type and H3.3B^K27R male third instar larvae stained for H4K16ac and DAPI. Arrow indicates the X chromosome. (B) Box plots of RNA-seq values for genes located on the X chromosome and the four main autosomal arms. The ratio of RPKM values in mutant versus wild type is plotted for each gene. H4K16 mutant data are from [45]. (C) Bar plot of H3.3B^K27R male viability for the six indicated genotypes. Data are plotted as the ratio of H3.3B^K27R males inheriting the chromosome indicated under each bar relative to its homologous wild type chromosome. Asterisks indicate statistical significance by chi-square test (p values as follows: msl-1: 9.6x10^-4, msl-2: 3.2x10^-5, msl-3: 4.4x10^-3, mle: 9.1x10^-4, CyO: 0.37, TM3: 0.69). The dashed line indicates the expected ratio of 1. n equals the total number of males scored. (D) Cartoon of the genomic location of the H3.3B^WT transgene relative to the endogenous H3 gene loci. (E) Bar plot of viability for homozygous H3.3B^K27R females and hemizygous H3.3B^K27R males inheriting the H3.3B^WT transgenic chromosome (purple) or the non-transgenic homologous chromosome (red). n equals the number of adults scored for each indicated genotype.