Fig 4. Histone modifications are diminished in H3.3^K27R flies.

Western blots of H3K27ac (A, E, G, J), H3K27me2 (C), and H3K27me3 (B, F, H, K) from H3.3B^K27R (A-C) and double H3.3^K27R (C, E, F) pharate adult males, 16–20 hour H3.3B^K27R male embryos (G, H), and H3.3B^K27R pharate adult males rescued by the H3.3B^WT transgene (J, K). (D, I, L) Bar plots depicting the average fold change in H3K27 PTM signal relative to wild type (WT). Each lane was also normalized to total H3 signal. Error bars represent standard deviation of at least two independent western blots.