Fig 6. H3.3B^K27R enhances Polycomb mutant phenotypes.

(A) Table quantifying the frequency of ectopic sex combs for the four indicated genotypes. P values were obtained from a Fisher’s Exact Test. (B) Bright field images of first and second thoracic legs from wild type and H3.3B^K27R hemizygous males that are also heterozygous for a Polycomb null allele. Arrows indicate the location where sex combs develop. (C) Bright field images of adult wings from wild type, H3.3B^K27R hemizygous males also heterozygous for a Polycomb null allele, and H3.3B^K27R heterozygous females also heterozygous for a Polycomb null allele. (D) Bar plots quantifying the severity of wing defects from the three indicated genotypes. * indicates p value of 2.7E-3, ** indicates p value 1.2E-6 (ordinal logistic regression). (E) Confocal images of wing imaginal discs stained for DAPI (blue) and Ubx (red) for the indicated genotypes. Arrows indicate the location of ectopic Ubx expression.