Skip to main content
. 2019 Jan 30;15(1):e1007578. doi: 10.1371/journal.ppat.1007578

Fig 4. miR-218-5p directly targets HMGB2 and CMPK1 to mediate vIRF1-induced cell motility, invasion and proliferation.

Fig 4

(A). Western-blotting of HMGB2 and CMPK1 expression in vIRF1-infected HUVECs transfected with a negative control mimic (NC of mimic; 20 nM), miR-218-5p mimic (miR-218-5p; 20 nM). (B). Western-blotting of HMGB2 expression in vIRF1-expressing HUVECs transfected with a mixture of siRNAs targeting HMGB2 (siHMGB2). (C). Plate colony formation assay of HUVECs treated as in (B). (D). Migration analysis of HUVECs treated as in (B). (E). Invasion analysis of HUVECs treated as in (B). (F). Western-blotting of CMPK1 expression in vIRF1-expressing HUVECs transfected with a mixture of siRNAs targeting CMPK1 (siCMPK1). (G). Plate colony formation assay of HUVECs treated as in (F). (H). Migration analysis of HUVECs treated as in (F). (I). Invasion analysis of HUVECs treated as in (F). (J). Western-blotting of HMGB2 expression in KSHV-infected HUVECs transfected with a mixture of siRNAs targeting HMGB2 (siHMGB2). (K). Western-blotting of CMPK1 expression in KSHV-infected HUVECs transfected with a mixture of siRNAs targeting CMPK1 (siCMPK1). (L). Migration and invasion analyses of HUVECs treated as in (J) and (K) at 6 h. The quantified results represent mean ± SD. Results are from three independent experiments, each with cubic (colony formation) or quadruple (migration and invasion) technical replicates, were performed. * P < 0.05, ** P < 0.01, and *** P < 0.001, Student's t-test.