Table 4.
Comparison of paraprotein and total Ig concentrations (g/L) between New Zealand laboratories.
| Assay | Gating | Sample 1 | Sample 2 | Sample 3 | Sample 4 | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Paraprotein * | Total IgA | Paraprotein | Total IgA | Paraprotein | Total IgA | Paraprotein | Total IgA | |||
| NZ Lab 1 | IH AGE | PD | 32 (47) | 43.9 | 9 | 10.2 | 28 | 26.2 | 10 | 6.4 |
| NZ Lab 2 | C CZE | PD | 33 (46) | 39.8 | 10 | 9.9 | 25 | 23.9 | 9 | 5.9 |
| NZ Lab 3 | C CZE | PD | 46 | 38.5 | 9 | 9.7 | 25 | 25.2 | 4 | 6.3 |
| NZ Lab 4 | C CZE | PD | 47 | 41.2 | 9 | 10.5 | 25 | 25.3 | 8 | 6.3 |
| NZ Lab 5 | C CZE | PD | 46 | 37.3 | 9 | 9.7 | 25 | 24.2 | 7 | 6.0 |
| NZ Lab 6 | C CZE | TS | 37 | 34.5 | 4 | 10.3 | 16 | 26.0 | 4 | 5.8 |
| NZ Lab 7 | C CZE | PD | 46 | 36.2 | 10 | 9.6 | 26 | 23.1 | 8 | 5.7 |
| NZ Lab 8 | AGE | PD | 44 | 36.7 | 8 | 8.7 | 25 | 22.8 | 9 | 5.8 |
| NZ Lab 9 | HR AGE | PD | 41 | 45.7 | 6 | 9.2 | 23 | 29.1 | 6 | 5.9 |
| NZ Lab 10 | C CZE | PD | 47 | 41.0 | 8 | 9.5 | 25 | 25.7 | 9 | 6.1 |
| Mean (g/L) | 42 | 39.5 | 8 | 9.7 | 24 | 25.2 | 7 | 6.0 | ||
| SD (g/L) | 5.9 | 3.5 | 1.9 | 0.5 | 3.2 | 1.8 | 2.1 | 0.2 | ||
| CV% | 14.0 (7.3) | 9.0 | 22.9 | 5.5 | 13.0 | 7.3 | 28.6 | 4.1 | ||
| CV% (C CZE All) | 13.0 (7.9) | 24.6 | 14.6 | 30.9 | ||||||
| CV% (C CZE PD only) | 12.4 (1.1) | 8.2 | 1.6 | 24.9 | ||||||
*
Sample 1 has two peaks.
The value for the first peak only was reported by Labs 1 & 2. Results in brackets reflect the combined concentration of two peaks. AGE, agarose gel electrophoresis; C, Capillarys®; CZE, capillary zone electrophoresis; HR, high resolution; PD; perpendicular drop; IH; in-house