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. Author manuscript; available in PMC: 2019 Feb 11.
Published in final edited form as: ACS Chem Biol. 2017 Nov 7;13(7):1844–1852. doi: 10.1021/acschembio.7b00748

Figure 2.

Figure 2.

Fluorescence imaging of labile copper pools in live HEK 293T cells with (a, g, m) CCF1, (b, h, n) CSF1, (c, i, o) CPF1, (d, j, p) Ctrl-CCF1, (e, k, q) Ctrl-CSF1, and (f, l, r) Ctrl-CPF1. Control cells (a–f) and cells incubated with (g–l) 100 μM CuCl2 or (m–r) 500 μM BCS in the growth medium for 12 h at 37 °C were stained with 5 μM dye for 30 min at 37 °C in DMEM. Scale bars: 40 μm. (s–x) Quantification of fluorescence intensity of cells stained with CCF1, CSF1, CPF1, Ctrl-CCF1, Ctrl-CSF1, and Ctrl-CPF1, respectively. Data were normalized to control cells and shown as average ± s.d. (CXF, n = 4; Ctrl-CXF, n = 3). ***P ≤ 0.001; two-tailed Student’s t test.