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. 2019 Jan 5;9(1):e3127. doi: 10.21769/BioProtoc.3127

Figure 5. Stimulation and analysis paradigm for estimating the RRP.

Figure 5.

A. Representative stimulation paradigm (above) and electrophysiological responses (below) of the 60 Hz/30 stimuli protocol necessary to determine the RRP. B. Representative EPSC recording during a 60 Hz stimulus train at a typical NMJ. C. Average cumulative EPSC amplitude plotted as a function of time. A line fit to the 18-30th stimuli was back extrapolated to time 0; this y-intercept value is divided by the mEPSC value to determine the size of the RRP.