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. Author manuscript; available in PMC: 2019 Feb 11.
Published in final edited form as: Cell Rep. 2019 Jan 29;26(5):1344–1356.e5. doi: 10.1016/j.celrep.2019.01.018

Figure 5. O-GlcNAcylation Antagonizes Phosphorylation of FNIP1.

Figure 5.

(A) In vitro O-GlcNAcylation of recombinant FNIP1-D-His6 and its non-phosphomutants. O-GlcNAcylation and interaction of FNIP1-D-His6 and its mutants with OGT-FLAG were assessed by immunoblotting.

(B) FNIP1-D-HA and its non-phosphomutants were co-expressed with either EV or OGT-V5 in HEK293 cells. Serine phosphorylation and O-GlcNAcylation of FNIP-1-D-HA and its mutants as well as their interaction with Hsp90 and OGT were assessed by IP and immunoblotting.

(C) Schematic representation of O-GlcNAcylation of FNIP1.