Figure 5.

Cell proliferation evaluation at 24 hour time point on unpatterned and 41 patterned surfaces (MARC chamber) with different ECM coatings and cell seeding densities. (A) Comparison of cell proliferation on non-coated surfaces at initial cell seeding density 3000 cells/cm² (A) and 10000 cells/cm² (B). Geometry size, isotropy and anisotropy are indicated. Arrows represent increase in geometry size. Mean values of non-collagen I and collagen I coated unpatterned surfaces are represented by dashed and dotted lines respectively. Data (n=20 for unpatterned surfaces and n=4 for patterned surfaces) were evaluated on outliers using Grubb’s test. Statistical analysis was performed using one-way ANOVA test with Tukey’s post-hoc test. Data represent mean±SD. No significant differences were observed.