Figure 3. Primase DnaG prevents helicase slippage.
(a) FRET efficiency traces of the G40P-DnaG complex on the 3GC substrate. Traces with successful unwinding showed no slippage events. (b) Fraction of all traces with protein activity with complete unwinding on different substrates (AT, 1GC, 2GC, 3GC) and at different enzyme conditions (G40P, G40p+DnaG) (n.s. denotes not significant, *p<0.05, ***p<0.001). (c) FRET efficiency traces of G40P unwinding the AT substrate with slippage events marked by stars before the actual unwinding events marked by the arrow at [ATP]=250 µM, 150 µM and 100 µM. (d) Semi-logarithmic plot of the average number of slippage events per total number of unwinding traces at different ATP concentrations. Addition of DnaG reduces the average number of slippage events before successful unwinding by a factor ≈ 2.5. Errors were estimated by bootstrapping analysis.
Figure 3—figure supplement 1. Unwinding traces of the 3GC DNA substrate by (G40P)6 in presence of DnaG.
