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. Author manuscript; available in PMC: 2019 Feb 11.
Published in final edited form as: Mol Reprod Dev. 2018 Feb 22;85(3):250–261. doi: 10.1002/mrd.22961

FIGURE 2.

FIGURE 2

Evaluation of porcine testicular cells transfected with GFP and TALENs by nucleofection. (a) Representative scatter plot of GFP transfected cells. P1 indicates germ cell gate, P3 the non-germ cell gate. (b) Immunofluorescence analysis of the germ cell population after FACS-sorting of GFP transfected porcine testicular cells. The merged image illustrates >80% germ cell transfection efficiency. (c) Representative scatter plots of porcine testicular cells after sham nucleofection (top panel) and after nucleofection with TALENs (lower panel). Note lower recovery of cells in the germ cell gate after nucleofection with TALENs. (d) Immunofluorescence analysis of the germ cell population after TALENs + GFP nucleofection and FACS-sorting. (e) Evaluation of NHEJ by Surveyor assay in TALENs transfected FACS-sorted germ cells, confirming NHEJ occurred in germ cells