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. Author manuscript; available in PMC: 2019 Feb 11.
Published in final edited form as: Mol Reprod Dev. 2018 Feb 22;85(3):250–261. doi: 10.1002/mrd.22961

TABLE 1.

Nucleofection of enriched porcine germ cells

Solution/
program
% of cell
recovery
% of GFP +
cells
% of UCH-
L1 + cells
% of GFP + UCH-L1 + cells
from recovered cells
% of GFP + UCH-L1 + from
recovered UCH-L1 + cells
% overall
yieldb
V/X-005 49.7 ± 5.72a,1 39.6 ± 2.161 52.9 ± 5.951 31.5 ± 7.671 46.5 ± 2.251 15.2 ± 1.991
L/X-001 54.8± 5.571 25.9 ± 7.741 50.5 ± 3.051 19.6 ± 10.831,2 37.1 ± 8.291,2 10.5 ± 5.011,2
B/U-030 33.1 ± 6.782 34.3 ± 2.871 43.6 ± 12.661 15.2 ± 7.622 39.1 ± 3.072 4.8 ± 2.172

Within each column, different numbers indicate statistically significant difference (p < 0.05).

a

mean ± SD; n = 3 for V/X-005 and B/U-030; n = 4 for L/X-001.

b

% of GFP+ UCH-L1+ cells from input cells subjected to nucleofection.