TABLE 2.

Transfection of porcine spermatogonia with TALENs

TALENs (µg)	Incubation temperature (°C)	% of cell recovery	% of GFP + cells	% of UCH- L1 + cells	% of GFP+ UCH- L1 + cells	% yield of GFP+UCH- L1 + cellsa	% of NHEJ	% yield of cells with NHEJb
5	37	49.9 ± 7.731	45.4 ± 3.211	62.7 ± 2.121	31.3 ± 4.251	16.1 ± 3.811	3.9 ± 1.101	2.0 ± 0.631
10	37	38.4 ± 0.711,2	36.5 ± 8.361,2	57.5 ± 1.352	27.7 ± 8.201,2	10.6 ± 2.961,2	11.5 ± 3.892,3	4.4 ± 1.412,3
25	37	17.6 ± 3.964	30.9 ± 9.102	53.7 ± 1.462	17.2 ± 2.062	3.0 ± 0.333	16.9 ± 0.493	3.0 ± 0.581,2
5	30	36.8 ± 4.642,3	50.1 ± 1.363	58.3 ± 0.492	30.9 ± 0.621	11.4 ± 1.341	8.1 ± 0.85 2	3.0 ± 0.461,2
10	30	34.7 ± 0.713	37.9 ± 2.642	54.7 ± 0.232	25.9 ± 0.081	9.0 ± 0.162	14.9 ± 1.703	5.2 ± 0.483
25	30	17.6 ± 1.274	41.2 ± 1.961,2	43.2 ± 0.113	23.3 ± 7.711,2	4.1 ± 1.073	17.7 ± 0.923	3.1 ± 0.062
After sortingc:							In germ cell gate	% NEHJ in non-germ cell gate
20	30	5.08 ± 2.51	55.94 ± 9.72	77.60 ± 11.99	42.19 ± 9.29	2.14 ± 1.06	5.76 ± 2.59	0.55 ± 1.10

Within each column, different numbers indicate statistically significant difference (p < 0.05), n = 4.

^a% of GFP+ UCH-L1+ cells from input cells subjected to nucleofection.

^b% of modified cells (NHEJ) from input cells subjected to nucleofection.

^cSeparate experiment.