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Targeted modifications in porcine germ cells detected by surveyor assay and MiSeq targeted sequencing
| Samplesa | % NHEJ by surveyor assay | MiSeq total count | MiSeq indel count | % Indels by MiSeq |
|---|---|---|---|---|
| 1 | 6 | 186,635 | 11,780 | 6.31 |
| 2 | 1.7 | 194,437 | 508 | 0.26 |
| 3 | 9.2 | 93,557 | 7,470 | 7.98 |
| 4 | 0 | 337,179 | 296 | 0.08 |
Samples 1–3 were transfected with 20 µg TALEN constructs, sample 4 is sham transfected control.
