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. 2018 Oct 18;20(3):328–337. doi: 10.1080/15384047.2018.1529101

Figure 5.

Figure 5.

FSTL1 is targeted by miR-137. (a) Predicted binding site in the 3ʹUTR of the FSTL1 gene with miR-137. (b) Mutant of FSTL1 3ʹUTR reporter plasmid was constructed. (c) Luciferase reporter assays were performed in 293T cells with co-transfection of indicated wild-type or mutant 3ʹUTR FSTL1 constructs and miR-137 or NC (negative control). *p < 0.05. (d) Relative expressions of miR-137 in MDA-MB-231, MDA-MB-468 and HCC38 cells with miR-137 or NC transfection. **p < 0.01. (e, f) RT-PCR (e) and western blot (f) analyses of FSTL1 expression in MDA-MB-231, MDA-MB-468 and HCC38 cells with miR-137 or NC transfection. **p < 0.01.