REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
FM4–64	Thermo Fisher Scientific	Cat#T13320
SYTOX Green	Thermo Fisher Scientific	Cat#S7020
Fosfomycin	MilliporeSigma	Cat#P5396
Bacitracin	MilliporeSigma	Cat#B0125
Penicillin V	MilliporeSigma	Cat#1504489
Cephalexin	MilliporeSigma	Cat#C4895
Cerulenin	MilliporeSigma	Cat#C2389
Rifampicin	MilliporeSigma	Cat#R3501
Experimental Models: Organisms/Strains
Bacillus subtilis PY79	Youngman et al., 1984	Tax. ID:1415167
Bacillus megaterium QM B1551	Dr. Peter Setlow at UConn Health	Tax. ID:545693
Bacillus thuringiensis subsp. israelenis	Bacillus Genetic Stock Center	4Q2; Tax. ID:339854
Bacillus pumilus BL8	Dr. Louise Temple at James Madison University	Tax. ID:1189615
See Table S1 for a complete list of strains used in this paper, including all the Bacillus subtilis PY79 variants	N/A	N/A
Oligonucleotides
See Table S2 for oligonucleotides	N/A	N/A
Recombinant DNA
Plasmid: pJLG38: GFPΩkan	This paper	N/A
Plasmid: pJLG82: amyE::PsspE(2G)-sspBΩcat	This paper	N/A
Plasmid: pJLG113: ileS-ssrAΩkan	This paper	N/A
Plasmid: pER226: rpsB-GFPΩkan	This paper	N/A
Software and Algorithms
JFilament	Smith et al., 2010	http://athena.physics.lehigh.edu/jfilament/
volumeestimator	Ojkic et al., 2016	https://elifesciences.org/articles/18657/figures#SD2-data
intensityestimator	This paper	Data S1
IMOD	Mastronarde, 1997	http://bio3d.colorado.edu/imod/; RRID: SCR_003297
TomoSegMemTV	Martinez-Sanchez et al., 2014	https://sites.google.com/site/3demimageprocessing/tomosegmemtv
Amira	Commercial software by Thermo Scientific (formerly FEI)	https://www.fei.com/software/amira-3d-for-life-sciences/; RRID: SCR_014305
EMAN2	Tang et al., 2007	http://blake.bcm.edu/emanwiki/EMAN2
Dynamo	Castaño-Díez et al., 2012	https://wiki.dynamo.biozentrum.unibas.ch/w/index.php/Main_Page