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. 2019 Feb 5;9:17. doi: 10.3389/fcimb.2019.00017

Table 1.

Sequencing summary.

INSTRUMENT
Manufacturer Illumina
Version HiSeq 2500
Slot used A
Basecalling pipeline -HiSeq Control Software 2.2.38 -RTA 1.18.61.0
-CASAVA-1.8.2
RUN
Mode HiSeq high output (HO) version 4
Number of cycles 2 × 150 + 7
Number of lanes 1
Flow cell ID HL7FHCCXX
Flow cell version HiSeq Flow Cell v4
Kit version HiSeq SBS Kit v4
Indexing Single-Indexing
Data output C 5.18 Gb
Data output (nvAG83 infected macrophage) 4.36 Gb
Data output (vAG83 infected macrophage) 4.36 Gb
SEQUENCING SPECIFICATION
Service Full lane
Error rate 1.5%
Q30 80%