Table 5.
Gene ontology (GO) categories enriched across virulent to non-virulent L. donovani intracellular stages.
| GO ID | Go term | P-value |
|---|---|---|
| UPREGULATED GO TERM IN VIRULENT PARASITE COMPARED TO NON-VIRULENT PARASITE | ||
| GO:0005524 | MF ATP binding | 0.003015992 |
| GO:0004514 | MF nicotinate-nucleotide diphosphorylase (carboxylating) activity | 0.008264463 |
| GO:0004516 | MF nicotinate phosphoribosyltransferase activity | 0.008264463 |
| GO:0004657 | MF proline dehydrogenase activity | 0.008264463 |
| GO:0006562 | BP proline catabolic process | 0.008264463 |
| GO:0009678 | MF hydrogen-translocating pyrophosphatase activity | 0.008264463 |
| GO:0019357 | BP nicotinate nucleotide biosynthetic process | 0.008264463 |
| GO:0004198 | MF calcium-dependent cysteine-type endopeptidase activity | 0.011468442 |
| GO:0005215 | MF transporter activity | 0.013800439 |
| GO:0015992 | BP proton transport | 0.016462677 |
| GO:0004427 | MF inorganic diphosphatase activity | 0.024595158 |
| GO:0009435 | BP NAD biosynthetic process | 0.024595158 |
| GO:0004672 | MF protein kinase activity | 0.030803132 |
| GO:0042026 | BP protein refolding | 0.032662417 |
| GO:0000166 | MF nucleotide binding | 0.04142298 |
| GO:0004674 | MF protein serine/threonine kinase activity | 0.048451125 |
| DOWNREGULATED GO TERM IN VIRULENT PARASITE COMPARED TO NON-VIRULENT PARASITE | ||
| GO:0051920 | MF peroxiredoxin activity | 0.000103507 |
| GO:0004601 | MF peroxidase activity | 0.000342495 |
| GO:0017061 | MF S-methyl-5-thioadenosine phosphorylase activity | 0.006010518 |
| GO:0004140 | MF dephospho-CoA kinase activity | 0.011986407 |
| GO:0015937 | BP coenzyme A biosynthetic process | 0.017927857 |
| GO:0050708 | BP regulation of protein secretion | 0.017927857 |
| GO:0004402 | MF histone acetyltransferase activity | 0.023835058 |
| GO:0006414 | BP translational elongation | 0.023835058 |
| GO:0009116 | BP nucleoside metabolic process | 0.029708199 |
| GO:0005643 | CC nuclear pore | 0.035547467 |
| GO:0019843 | MF rRNA binding | 0.041353051 |
GOseq was used to identify enriched GO categories between the virulent and non-virulent intracellular amastigotes at a p-value cutoff of < 0.05. For the difference between both the parasites, up- and down-regulated genes were considered separately. The category for each enriched GO term is indicated (BP, biological process; MF, molecular function; CC, cellular component).