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. 2019 Feb 5;10:153. doi: 10.3389/fmicb.2019.00153

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Copyright © 2019 Zhao, Wu, Xu, Ma, Ding, Bai, Rong, Zhang, Li and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification and secondary structural analysis of UreA-His mutants. (A) Seven purified mutants of UreA-His. (B) CD spectra of UreA-His and its mutants. UreA-His mutants were purified in the same procedure as Hsp60, except that the buffer used was changed to PBS (pH 7.4).