Table 1.
Primers used in this study.
| Primer | Description or sequencea |
|---|---|
| H-F | ATGGCAAAAGAAATCAAATTTTC |
| H-R | TTACATCATGCCACCCATGCC |
| UA-GN-F | GTCTCGGAATTCAAACTCACCCCAAAAGAGTTAG |
| UA-GN-R | GCTGCGCTCGAGTTACTCCTTAATTGTTTTTACATAG |
| UB-GN-F | GCACGGGAATTCAAAAAGATTAGCAGAAAAGAAT |
| UB-GN-R | CATGGCCTCGAGCTAGAAAATGCTAAAGAGTTGC |
| UA-GC-R | CGGTCGGAATTCCTCCTTAATTGTTTTTACATAGT |
| UB-GC-R | TCGTAGGAATTCGAAAATGCTAAAGAGTTGCGCCAA |
| GST-F | GCTACGGAATTCATGTCCCCTATACTAGGTTA |
| GST-R | TGATGGCTCGAGTTAATCCGATTTTGGAGGATGGT |
| UA-H-F | CGAAGTCCCATATGAAACTCACCCCAAAAGAGTTAG |
| UA-H-R | GCTGCGCTCGAGCTCCTTAATTGTTTTTACATAG |
| UB-H-F | CGAAGTCCCATATGAAAAAGATTAGCAGAAAAGAAT |
| UB-H-R | CATGGCCTCGAGGAAAATGCTAAAGAGTTGC |
| UreA-Y15A-F | CGAAGTCCCATATGAAACTCACCCCAAAAGAGTTA GACAAGTTGATGCTCCACGCTGCTGG |
| UreA-K22A-F | CGAAGTCCCATATGAAACTCACCCCAAAAGAGTTAG ACAAGTTGATGCTCCACTATGCTGGAGAATTGGCT AAAGCTCGCAAAG |
| UreA-R23A-F | CGAAGTCCCATATGAAACTCACCCCAAAAGAGTTAG ACAAGTTGATGCTCCACTATGCTGGAGAATTGGCTA AAAAAGCCAAAGAAAAAGGC |
| UreA-E25A-F | CGAAGTCCCATATGAAACTCACCCCAAAAGAGTTAG ACAAGTTGATGCTCCACTATGCTGGAGAATTGGCTA AAAAACGCAAAGCTAAAGGC |
| UreA-K26A-F | CGAAGTCCCATATGAAACTCACCCCAAAAGAGTTAGA CAAGTTGATGCTCCACTATGCTGGAGAATTGGCTAA AAAACGCAAAGAAGCTGGCATTAAGC |
| UreA-K66A-F | GCGCACTCTTTTAGCTCCGGATGATGTGATGG |
| UreA-K66A-R | CCATCACATCATCCGGAGCTAAAAGAGTGCGC |
| UreA-D68A-F | GCACTCTTTTAAAACCGGCTGATGTGATGGATGGC |
| UreA-D68A-R | GCCATCCATCACATCAGCCGGTTTTAAAAGAGTGC |
aRestriction sites on the primers are underlined. Mutated bases are marked with bold font and underlined with double lines.