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. 2019 Feb 5;10:97. doi: 10.3389/fimmu.2019.00097

Figure 2.

Figure 2

RANKL overexpression exacerbates TNF-driven arthritis. Assessment of arthritis progression was conducted in Tg197/TgRANKL mice (Tg197/Tg5516, Tg197/Tg5519), Tg197 and sex-matched control littermates (WT, Tg5516, and Tg5519) till the 6th week of age. (A) Body weight curves (n = 10 per genotype), (B) clinical arthritic scores (n = 10–15 per genotype), (C) histological arthritic score (n = 10–15 per genotype), and (D) representative ankle joint sections from each genotype (n = 10–15) at the 6th week of age stained with hematoxylin/eosin (H&E), TRAP for osteoclasts and Toluidine Blue (TB) for articular cartilage destruction. Arrows at H&E indicate focal pannus invasion into subchondral bone regions. Scale bars: 300 μm at H&E and TRAP; 150 μm at TB. Data represent mean values ± SEM. One-Way ANOVA and Tukey post-hoc test was performed. Asterisks mark statistically significant difference (*p < 0.05, **p < 0.01, ***p < 0.001).