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. 2019 Feb 5;10:65. doi: 10.3389/fneur.2019.00065

Table 1.

Oligonucleotide primers for RT-qPCR analysis.

Gene Genbank accession number Primer sequence
ApoE NM_001270681.1 F: 5′-ACCTAATGGAGAAGATACA-3′
R: 5′-GAGAATCTTTATTAAGCAAGG-3′
Probe: 5′-FAM-AACTCCATTGCCTCCACCAC-TAMRA-3′
Apoa1 NM_012738 F: 5′-CGATCAGATGCGCGAGAAC-3′
R: 5′-TACTCGATCAGGGTAGGGTGGTT-3′
Probe: 5′-FAM-CCCAGCGCCTGACCGAGATCAA-TAMRA-3′
PDK4 NM_053551 F: 5′-GCTCACACAAGTCAATGGAAAATT-3′
R: 5′-ATGTGGTGAAGGTGTGAAGGAA-3′
Probe: 5′-FAM-CCAGGCCAACCAATCCACATCGTG-TAMRA-3′
Annexin A7 NM_130416 F: 5′-TTGTGGATGTCGTGTCTA-3′
R: 5′-GGCATCATAGTATGTAGGAG-3′
probe: 5′-FAM-CGTTCCAATGACCAGAGGCA-TAMRA-3′
cPLA2 NM_133551 F: 5′-AGATCCTTATCAGCACAT-3′
R: 5′-CACAGGGTTTATATCATTATTG-3′
probe: 5′-FAM-TTGTTCGCTTCCTGCTGTCA-TAMRA-3′
ZnT-1 NM_022853 F: 5′- CGTTGTTGTGAATGCCTTGGT-3′
R: 5′-GGGTTCACACAAAAGTCGTCTTC-3′
probe:: 5′-FAM-TTCTACTTTTCCTGGAAGGGTTGTA-TAMRAM-3′
ZnT-3 NM_001013243 F: 5′- TGGGCGCTGACGCTTACT-3′
R: 5′- GTCAGCCGTGGAGTCAATAGC-3′
probe:: 5′-FAM-ACCACGTTGCCTCCGCACACCT-TAMRAM-3′
ZnT-4 NM_172066.1 F: 5′- GCTGAAGCAGAGGAAGGTGAA−3′
R: 5′- TCTCCGATCATGAAAAGCAAGTAG−3′
probe:5′-FAM-CAGGCTGACCATCGCTGCCGT-TAMRA−3′
ZnT-5 NM_001106404.1 F: 5′- CCAGCACATGTCTGGCCTAA−3′
R: 5′- TTTGCAGTACTTCATGGATTCCA−3′
probe: 5′-FAM-CACTGGCTTCCACGATGTCCTGGCTAT– TAMRA-3′
ZnT-6 NM_001106708.1 F: 5′- CGGCATTATCCCAGGACTCA−3′
R: 5′-CCAGCAAGATCGATCAGAACAA−3′
probe: 5′-FAM- TTCTTGCCCCGCATGAACCCG -TAMRA-3′
ZnT-7 XM_001073594.1 F: 5′- TTGGGATCCGCGTCTGA−3′
R: 5′- CCCTCTAGAAGTGACTCGGTATGG−3′
probe: 5′-FAM-TCGTCTCTGCTGTCACTGCCGCC–TAMRA- 3′