Figure 2.

Inhibition of CheR1 by c-di-GMP in methylating MCPs requires MapZ. Methylation of eight MCPs by CheR1 demonstrated by in vitro methyltransferase assay with the methylated MCPs visualized by radio-autography. Shown radio-autography images (A–C) are representative of two independent in vitro methyltransferase assay. The membrane fraction-containing chemoreceptor and [³H]-Ado-Met were used as the substrate and co-substrates for CheR1, respectively. The amount of membrane fraction was normalized according to the corresponding protein expression level.