Figure 3.

Chemotaxis and energy taxis assays demonstrating that MapZ affects the taxis toward inorganic phosphate, malate, and energy source. (A) Capillary chemotaxis assay of P. aeruginosa strains attracted toward 10 mM inorganic phosphate (Pi). The data were the means of three replicates and were normalized with the number of bacteria that swam into buffer-containing capillaries (Two-tailed t-test, ^***P < 0.001). (B) Capillary chemotaxis assays of P. aeruginosa strains attracted toward 5 mM malate. The data were the means of three replicates and were normalized with the number of bacteria that swam into buffer-containing capillaries (Two-tailed t-test, ^****P < 0.0001). (C,D) Energy taxis of P. aeruginosa strains on the minimal medium plates that contained 50 mM glucose as the sole carbon and energy source. The area of the swimming zone was quantified and compared in (D). The data were the means of three replicates (Two-tailed t-test, ^*P < 0.05).