Table 1.
Overview of experimental 3D culture design.
| Method | Subgroup | Initial seeding density | Medium | Coating type |
|---|---|---|---|---|
| Hanging drop technique (HD) | – | 10,000 cells/well | RPMI 1640 | – |
| Inlay method (IM) | IM1 | 10,000 cells/well | Methylcellulose 0,3% | – |
| IM2 | RPMI 1640 | Cell repellent surface | ||
| IM3 | Methylcellulose 0,3% | Cell repellent surface | ||
| On top method (OM) | OM1 | 10,000 cells/well | RPMI 1640 | Methylcellulose 1,5% |
| OM2 | Sea plaque agarose 1,5% | |||
| Sandwich method (SM) | SM1 | 10,000 cells/well | RPMI 1640 + Matrigel 10% |
Methylcellulose 1,5% |
| SM2 | Sea plaque agarose 1,5% | |||
| SM3 | RPMI 1640 + BME 10% |
Sea plaque agarose 1,5% | ||
| SM4 | 5,000 cells/well | Sea plaque agarose 1,5% |