FIGURE 10.

BnABI3 is involved in lateral root (LR) development and confers a novel interaction of ABA and auxin signaling in roots. (A) Root phenotype and (B) LR per 1 cm primary root of 7-day-old WT, gs1, and abi3-6 mutant and 35S::BnABI3 transgenic plants. (C) After gravitropic stimulation to induce the synchronized initiation of LR primordia at the surface of bending roots, LR primordium stages from I to VIII. (D) Phenotypic analysis of LR emergence was achieved by synchronizing LR formation with a gravity stimulus for 16 and 36 h. Compared with WT (Col-0) the transgenic plants showed increased LR emergence. Data are shown as percentages, and the error bars represent SD, n = 11–15. At least 34 total LR primordia were observed for each plant. (E,F) LR per 1 cm primary root of 3-day-old WT, gs1, and abi3-6 mutant and 35S::BnABI3 transgenic plants exposed to various concentrations of IAA and NPA treatments for 10 days. Bar indicates 1 cm. Values are given as mean ± SD, n = 4. ^∗p < 0.05 by Kruskal–Wallis test.