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. 2019 Feb 5;10:67. doi: 10.3389/fpls.2019.00067

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Copyright © 2019 Xu and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BnABI3 overexpression confers ER stress tolerance. (A) The sensitivity of WT, gs1, and abi3-6 mutant and gs1 and proAtABI3::BnABI3 transgenic plants to ER stress. Photographs of 10-day-old Arabidopsis seedlings grown on MS medium supplied without or with 80 ng/ml tunicamycin (TM) are shown. (B) The percentage of Green-Big (G-B), Green-Small (G-S), and Yellow-Small (Y-S) plants were calculated. The difference between the WT and each transgenic line is shown. Bars depict SD (n = 3). (C) RT-qPCR analysis of BIP1, BIP3, and PID1 expression levels in WT, gs1, and abi3-6 mutant and gs1 and proAtABI3::BnABI3 transgenic plants were either left untreated or exposed to TM. A group of 10 plants was measured for each genotype. Values are given as mean ± SD, n = 3. ^∗p < 0.05 by Mann–Whitney test.