Table 1.
Primer sequences used for RT-qPCR.
| Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|
| Cldn10b | GGAGTTCCCCTCCATGCT | GCAAAAATGGAACCGAAAAA |
| Cldn14 | GTCCAGCTCCTAGGCTTCCT | CATCCACAGTCCCTTCAGGT |
| Cldn16 | GTTGCAGGGACCACATTAC | GAGGAGCGTTCGACGTAAAC |
| Cldn19 | GGTTCCTTTCTCTGCTGCAC | CGGGCAACTTAACAACAGG |
| Cnnm4 | TCTGGGCCAGTATGTCTCTG | CACAGCCATCGAAGGTAGG |
| Fxyd2 | TCAGCCTTTCTTGTGACTGG | GGTCTTCCTGTGGCCTCTACT |
| Hprt | TTGCTGACCTGCTGGATTAC | AGTTGAGAGATCATCTCCAC |
| Slc12a1 | CACATGGTCTTCCACTGTGGTT | GGCTCCTCCACACAGGCTC |
| Slc12a3 | CTTCGGCCACTGGCATTCTG | GATGGCAAGGTAGGAGATGG |
| Slc41a1 | CATCCCACACGCCTTCCTGC | CGGCTGGCCTGCACAGCCAC |
| Slc41a3 | TGAAGGGAAACCTGGAAATG | GGTTGCTGCTGATGATTTTG |
| Trpm6 | CTTCACAATGAAAACCTGCCC | AAAGCCATGCGAGTTATCAGC |
| Trpm7 | GGTTCCTCCTGTGGTGCCTT | CCCCATGTCGTCTCTGTCGT |
Cldn10b, claudin 10b; Cldn14, claudin 14; Cldn16, claudin 16; Cldn19, Claudin 19; Cnnm4, cyclin M4; Fxyd2, FXYD-domain containing 2; Hprt, hypoxanthine-guanine phosphoribosyltransferase; Slc12a1, solute carrier family 12 member 1; Slc12a3, solute carrier family 12 member 3; Slc41a1, solute carrier family 41 member 1; Slc41a3, solute carrier family 41 member 3; Trpm6, transient receptor potential melastatin type 6; Trpm7, transient receptor potential melastatin type 7.