Fig. 6.

TP53^R248L promotes immune cell infiltration by upregulating CCL2 and TNFA. a qPCR analysis showing expression of chemoattractive cytokines in TP53^R248L-overexpressing U87MG cell line. b qPCR analysis confirming shRNA-mediated knockdown of CCL2 in TP53^R248L-overexpressing U87MG cell line. shNT indicates non-targeting control shRNA. c–f Relative infiltration rate of BV2 microglia, HL60-derived monocytes, neutrophils, and macrophages grown in CM from a TP53^R248L-overexpressing U87MG cell line with or without CCL2 knockdown, and control counterpart cells. The bar graphs represent mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; n = 3)