Figure 3.

Effects of APS on expression of key proteins in the TLR4 signaling pathway, NF-κB and RIG-I in H₂O₂-pretreated MEFs. (A–F) Cells were treated for varying times (0, 3, 6, 9 and 12 h) with 20 µg/mL APS after stimulation with 250 µM H₂O₂ for 12 h. Proteins levels were determined by Western blotting, and the band intensities were analyzed using image analysis software. (G) Cells were stimulated with 250 µM H₂O₂ for 12 h. The MEFs were incubated in the presence or absence of a TLR4-neutralizing antibody (20 μg/mL) for 1 h and then treated with 20 μg/mL APS for 6 h, and the T-AOC was determined. The data are presented as the mean ± SD (n = 3 per group), and significance relative to the control group was determined using Duncan’s multiple-range test; *P < 0.05 and ***P < 0.001.