Fig. 4.

Cell cycle pathway is an important regulatory target of c-Myc during myoblast differentiation. a Results of the ChIP-qPCR validation of the 12 c-Myc-bound genes shown in Fig. 4c. NC represents a genomic region without an identified c-Myc-binding peak. b Relative mRNA expression of the indicated genes after c-Myc knockdown in primary myoblasts. The results are shown as the mean ± sem of three independent experiments. Independent sample t-test was performed to determine the significant differences between the groups. *p < 0.05; **p < 0.01