Fig. 7.

c-Myc-repressed miRNAs are involved in the regulation of c-Myc expression during myoblast differentiation. a Schematic representation of four miRNAs and c-Myc 3' UTR target region duplexes. b Relative miRNA expression of 4 miRNAs during myoblast differentiation. c Relative luciferase activity of the c-Myc 3' UTR reporter constructs after transfection with selected miRNA mimics. d c-Myc protein expression after transfection with selected miRNA mimics in primary myoblasts. The results are shown as the mean ± sem of three independent experiments. One-way analysis of variance (ANOVA) followed by Dunnett’s test was performed to determine the significant differences between the groups. *p < 0.05; **p < 0.01; ns, no significant difference