Fig. 8.

c-Myc is associated with lincRNA loci, and certain lincRNAs regulate myoblast proliferation and differentiation. a, b MyomiR-lincRNA interaction target networks. MyomiRs shown in red play positive roles in myoblast differentiation. MyomiRs shown in green play negative roles in myoblast differentiation. c Two selected lincRNAs and their target MyomiRs. d Results of the ChIP-qPCR validation of c-Myc binding to the linc-2949 and linc-1369 promoters between GM and DM. e Relative lincRNA expression after c-Myc knockdown in primary myoblasts. f Relative expression of linc-2949 and linc-1369 during myoblast differentiation. g Relative linc-2949 and linc-1369 expression in chicken tissues. h Relative luciferase activity of linc-2949 and linc-1369 reporter constructs after transfection with the indicated miRNA mimics. i CCK-8 assay was performed to assess the effect of si-linc-2949 and si-linc-1369 on myoblast proliferation. j Relative mRNA expression of the differentiation marker genes after transfection with si-linc-2949 and si-linc-1369. k Schematic of c-Myc-induced regulatory network in myoblast proliferation and differentiation and muscle fibre hypertrophy. The results are shown as the mean ± sem of three independent experiments. In f, h–j, ANOVA followed by Dunnett’s test was used. In e, independent sample t-test was performed to determine the significant differences between the groups. *p < 0.05; **p < 0.01; ns, no significant difference