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. 2019 Feb 11;10:693. doi: 10.1038/s41467-019-08530-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The NADP⁺/NAD⁺ ratio is associated with the cell sensitivity to poly(ADP-ribose) polymerase (PARP) inhibitor. a The sensitivity of 20 ovarian cancer cell lines to olaparib. Twenty ovarian cancer cell lines were treated with olaparib at the indicated doses. Cells were examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assays. Based on the sensitivity to olaparib, the cell were classified into four groups. The data were summarized from three independent experiments. Data are presented as mean ± SD. b Spearman’s correlation analysis was performed between DNA damage-induced PARylation levels and the IC50 of olaparib. DNA damage-induced endogenous PARylation levels were summarized from the western blotting in Supplementary Figure 3. IC50 of olaparib was calculated from the MTT assays. DNA damage-induced endogenous PARylation levels correlated with IC50 of olaparib (left) and PARylation levels in the olaparib-sensitive cells are markedly lower than that in the olaparib-insensitive cells (right). c The expression level of PARP1 is not associated with the sensitivity to olaparib. PARP1 expression levels were summarized from western blotting results in Supplementary Figure 4 by using ImageJ. No correlation was seen between PARP1 expression levels and IC50 of olaparib (left); and no difference was observed in the PARylation levels between the olaparib-sensitive cells and the olaparib-insensitive cells (right). d, e Correlation analysis of NAD⁺ and NADP⁺ concentrations and cell sensitivity to olaparib. NAD⁺ and NADP⁺ concentrations were measured in 20 ovarian cancer cell lines. Both NAD⁺ and NADP⁺ concentrations are associated with the sensitivity to olaparib (left). The NAD⁺ concentrations in the olaparib-sensitive cells are markedly higher than that in the olaparib-insensitive cells (d, right). And the NADP⁺ concentrations in the olaparib-sensitive cells is markedly lower than that in the olaparib-insensitive cells (e, right). f Sensitivity of ovarian cancer cells to olaparib is associated with the NADP⁺/NAD⁺ ratio. The NADP⁺/NAD⁺ ratio was calculated. NADP⁺/NAD⁺ ratio shows significant correlation with sensitivity of ovarian cancer cells to olaparib. p Value of Spearman’s correlation was calculated by R function, cor.test() (b–e, left; f). Statistical significance of the difference between the olaparib-sensitive cells and olaparib-insensitive cells was analyzed using two-tailed unpaired Student’s t tests (b–e, right)