Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 11;9:1796. doi: 10.1038/s41598-018-34828-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Immunostaining and automated cell segmentation of calcium time-series hNESC differentiated in chips of the OrganoPlate inside the Pelican. (A) Top view of an entire chip in the OrganoPlate showing differentiated wild type neurons (K7 cell line) immunostained for nuclei with Hoechst (blue), TUBβIII (green) and tyrosine hydroxylase (red); scale bar 300μm. (B) Enlarge top (B) and front (C) views of selected area; scale bar 50μm. (D) Differentiation efficiency of neurons positive for TUBβIII and tyrosine hydroxylase. (E) Mean fluorescence frame of a calcium time-series of WT population with segmented regions of interest corresponding to individual neurons and (F) their corresponding fluorescence traces. (G) Mean fluorescence frame of a calcium time-series of PINK1 mutants with segmented regions of interest corresponding to individual neurons and their corresponding fluorescence traces (H).