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. 2019 Feb 11;9:1734. doi: 10.1038/s41598-018-37584-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Self-aggregation of different α-synuclein constructs observed by FCCS. (a) Representative auto-correlation (red: Cy5-labelled, cyan: Oregon green-labelled); and cross-correlation (orange) curves for αS-8 at different incubation times. (b) Average diffusion time of dual-labelled aggregates for self-aggregation of αS-1 (black), αS-2 (orange), αS-4 (cyan), and αS-8 (red) at different incubation times. (c) Diffusion time of single-color aggregates labelled with Cy5. (d) Diffusion time of single-color aggregates labelled with Oregon green. Note that the non-monotonic fluctuations in diffusion time reflect the statistical variability and are not significant. Error bars represent s.e.m from 2–5 replicates of 50 measurements each.