Fig. 3.

Th17 cell program and pStat3 Y705 are defective in Hectd3-deficient T helper cells during EAE. a Representative flow cytometry analysis of intracellular IL-17A and GM-CSF in CD4⁺ T cells from the CNS of Hectd3^−/− and wild-type (WT) mice, 13 days following EAE induction. b Frequencies and absolute numbers of IL-17A⁺, GM-CSF⁺, IFNγ⁺, and IL-17A⁺GM-CSF⁺ CD4⁺ T cells in the CNS of Hectd3^−/− and WT mice, 13 days following EAE induction; n = 5 per group, from three independent experiments. c, d Representative flow cytometry histograms, frequencies, and MFIs of CCR6, CD29, and IL-23R in draining lymph node CD4⁺ T cells from the indicated groups of mice 10 days following EAE induction; data (n = 6) are representative of three independent experiments. e Representative flow cytometry analysis of RORγt⁺ T-bet⁺ CD4⁺ T cells in the CNS of Hectd3^−/− and WT mice, 13 days following EAE induction, n = 6 per group from three independent experiments. f Absolute numbers of RORγt⁺ CD4⁺ T cells and RORγt⁺ T-bet⁺ CD4⁺ T cells in the CNS of Hectd3^−/− and WT mice, 13 days following EAE induction. g Representative immunoblot of pStat3 Y705 and Stat3 protein level in CD4⁺ T cells isolated from draining lymph nodes of Hectd3^−/− and WT mice, 13 days following EAE induction, from five independent experiments. Data are mean ± SEM; p value was obtained from Student’s t test. Source data are provided as a Source Data file. Gating strategy is shown in Supplementary Fig. 9