Fig. 4.

Hectd3-deficient Th17 cells have reduced pathogenicity. EAE disease scores (mean ± SEM) of WT recipients transferred with in vitro IL-23 re-stimulated CD4⁺ T cells from either Hectd3^−/− or WT mice, induced with EAE, as indicated in Materials and methods. Details on induction of passive EAE are provided in Materials and methods. Clinical scoring of recipient mice was performed using criteria listed in Material and methods, 5 days following adoptive/passive transfer of in vitro reactivated CD4⁺ T cells into recipient mice; n = 5 per group from two independent experiments. Mann–Whitney two-tailed test was used for statistical analysis of EAE clinical scores. Source data are provided as a Source Data file