Table 1.
List of the oligonucleotide primers used.
| Gene | Accession no. | Forward primer 5′-3′ | Reverse primer 5′-3′ | Amplicon length (bps) |
|---|---|---|---|---|
| GAPDH | NM_002046.5 | AGGGCTGCTTTTAACTCTGGT | CCCCACTTGATTTTGGAGGGA | 205 |
| NANOG | NM_024865.3 | TTTGTGGGCCTGAAGAAAACT | AGGGCTGTCCTGAATAAGCAG | 115 |
| OCT-4 | NM_002701.5 | CTTTCTCAGGGGGACCAGTG | GGGACCGAGGAGTACAGTGC | 102 |
| RUNX2 | NG_008020.1 | GCCAGGGTCTAGGAGTTGTT | ACCCACCACCCTATTTCCTG | 212 |
| ALP | NM_000478.5 | ATGAAGGAAAAGCCAAGCAG | ATGGAGACATTCTCTCGTTC | 276 |
| SP7 (OSX) | NM_152860.1 | CCTCTGCGGGACTCAACAAC | AGCCCATTAGTGCTTGTAAAGG | 127 |
| DSPP | NM_014208.3 | TTTGGGCAGTAGCATGGG | CCATCTTGGGTATTCTCT | 181 |
| PPAR-γ2 | NM_138712.3 | GAACGACCAAGTAACTCTCC | CGCAGGCTCTTTAGAAACTCC | 137 |
| LPL | NM_000237.2 | ACGGCATGTGAATTCTGTGA | GGATGTGCTATTTGGCCACT | 200 |
Abbreviations: GAPDH, Glyceraldehyde-3-phosphate dehydrogenase; OCT-4, Octamer-binding transcription factor 4; RUNX2, Runt-related transcription factor 2; ALP, Alkaline phosphatase; SP7(OSX), Sp7 Transcription factor; DSPP, Dentin Sialophosphoprotein; PPAR-γ2, Peroxisome proliferator-activated receptor; LPL, Lipoprotein lipase.