Fig. 6.

High copy suppressors of ER stress-induced death of cnb1∆ cells. a, b Upon overexpression on 2 µ plasmids, three mitochondrial genes, AIM19, MRM1, and MRX9 rescue cnb1∆ and ire1∆ cells from ER stress-induced death and ROS. Viability and ROS after 5 h incubation with 0.5 µg/mL tunicamycin was determined as described in Fig. 1b, c legends. The results are expressed as a percent of untreated cells. c Overexpression of Aim19, Mrm1, and Mrx9 increase O₂ consumption in wild-type, ire1∆, and cnb1∆ cells. d High copy suppressors of ER stress-induced death increase ETC component levels. Western blot of wild-type, cnb1∆, and ire1∆ cells with and without high copy suppressors.