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. 2019 Feb 11;9:1739. doi: 10.1038/s41598-018-38056-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative viscosity blinded study. Here, genomic V. cholerae DNA that underwent the 65 °C heating of LAMP is represented as (+) heat, no genomic V. cholerae DNA that underwent the 65 °C heating of LAMP is (−) heat, genomic V. cholerae DNA that did not undergo heating is (+) no heat, and no genomic V. cholerae DNA that did not undergo heating is (−) no heat. (A) A 2% agarose gel of a representative blinded test to show DNA amplification in the positive sample. (B) Relative PD results show the experimental sample (+) heat is statistically significantly more viscous than control samples (****p-value < 0.0001). Nine PD measurements were made for each sample to allow for statistical comparison.