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. 2019 Feb 11;9:1739. doi: 10.1038/s41598-018-38056-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Measuring LAMP amplification from V. cholerae whole cells. Cells were spiked into LAMP reactions at concentrations ranging from 10⁰–10⁵ cells/reaction. The change in (A) EvaGreen/Rox is significant at 10² (**p < 0.01), 10³, 10⁴, and 10⁵ (***p < 0.001) cells/reaction. (B) The change in diffusivity measurements show an increasing trend as a function of starting cell concentration with significance at 10⁰ (**p < 0.01), 10¹, 10² (***p < 0.001), 10³, 10⁴, and 10⁵ (****p < 0.0001) cells/reaction. Statistical analysis was a one-way ANOVA with Dunnett’s post-hoc relative to NTC (n = 3).