Fig. 1.

Deletion of TRADD improves the survival of Ripk1^−/−Ripk3^−/− into young adulthood. a Actual and expected frequencies of pups at weaning age from intercrosses of Ripk1^+/−Ripk3^−/− Tradd^−/− mice. Chi square analysis shows no significant difference between actual and expected (p = 0.472). b Kaplan–Meier plot showing survival of mice of indicated genotypes. Logrank tests shows a significant increase in survival of Ripk1^−/− Ripk3^−/− Tradd^−/− mice, compared to Ripk1^−/− Ripk3^−/− mice (p < 0.0001) but significantly less than WT mice (p < 0.0001). c Weights of Ripk1^−/− Ripk3^−/− Tradd^−/− mice and littermate controls over time presented as mean ± SEM, *p < 0.05 as determined by Student’s t test. d Three week old and two month old Ripk1^−/− Ripk3^−/− Tradd^−/− mice (*) with littermate controls (n = 5 independent experiments). e Hematoxylin and eosin (H&E) and cleaved caspase 3 (CC3) staining of the large intestine of indicated genotypes (n = 3 independent experiments). Scale bars are 50 μm