Fig. 2.

Ubqln4 represses GC cell proliferation in vitro and in vivo. Stable MKN45 and BGC-823 cell lines were generated by infection with Ubqln4 or control lentiviruses for 24 h and screening in 2 μg/ml puromycin for 2 weeks. a Indicated cell lines were plated into 96-well plates and cell viability was examined every 24 h by MTT assay. Each point represents mean ± SD of duplicates (n = 8). Student’s t-test; **P < 0.01, ***P < 0.001. b Colony-formation assay in MKN45 and BGC-823 stable cell lines expressing Ubqln4 compared with controls after 14 days of culture. Colony numbers in triplicates in three experiments were counted. Values are presented as mean ± SD. ***P < 0.001. c Xenograft tumors were established by injection of MKN45 cells stably expressing Ubqln4 compared with the controls (n = 5, group). Representative images of nude mice are shown. Tumor mass volume was every 4 days after injection. Measurements were repeated three times. Values are presented as mean ± SD. Student’s t-test. **P < 0.01. d Images of tumor xenografts from the indicated groups. Tumor weight was measured after mice was killed at the 28th day. Values are presented as mean ± SD. Student’s t-test. **P < 0.01