Fig. 4.

Ubqln4 induces alterations of the p53 signaling pathway in MKN45 cells. a Gene expression changes (log2FC) of 27 selected genes representing the p53 signaling pathway and DEGs in MKN45 cells with overexpression of Ubqln4 using quantitative RT-PCR. All quantitative RT-PCR experiments were performed in triplicate. Student’s t-test. **P < 0.01, ***P < 0.001, ****P < 0.0001. b Western blot analysis for the indicated proteins in MKN45 cells infected with Ubqln4 lentivirus at various concentrations (1:0.25, 1:0.5, 1:1, 1:2, and 1:4) for 48 h. c Western blot analysis for the indicated proteins in MKN45 cells infected with Ubqln4 lentivirus alone or together with Ubqln4-sh1 or Ubqln4-sh2 lentivirus as indicated. MKN45 cells infected with control lentivirus were used as controls. d, e MKN45 cells were treated with (d) etoposide (ETO) or (e) camptothecin (CPT) at the indicated concentrations for 24 h and Western blot was performed with the indicated antibodies