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. 2019 Feb 5;10:29. doi: 10.3389/fphar.2019.00029

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Copyright © 2019 Wan, Xu, Song, Liu, Wang, Zhao, Jiang, Liu, Zeng and Tu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Direct target protein identification and neuroprotective pathway-gene network analysis of CEE. (A) The potential target proteins were captured by the CEE-coated beads, separated by 10% SDS-PAGE, and then visualized by silver staining. (B) The expression of YBX1, HMGB1, HSP70, and Histone H2B were detected by western blotting. (C) 150 potential target proteins were identified by LC-MS/MS and classified into 6 GO terms in biological processes. (D) 150 potential target proteins were classified into 10 terms in reactome pathways. (E) The pathway-gene network comprised of 150 potential target proteins. The analysis was based on the Homo sapiens gene annotation databases, more than two genes in each term and P-value < 0.05.