Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 7;176(4):729–742.e18. doi: 10.1016/j.cell.2018.12.009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure S1 — Functional Characterization of Rare Human Variants in SEMA3A-G, Related to Figure 1

(A) Total expression of C-FLAG-tagged SEMA3A-G by ELISA analysis (A.U., arbitrary units).

(B) Western blotting of total cellular and secreted SEMA3A-G.

(C) Dimerization analysis using reducing and non-reducing western blotting of total cellular and secreted SEMA3G.

(D) Collapse efficiency was assessed by counting the proportion of collapsed cells 30 min following addition of the indicated WT Semaphorin to the culture medium.

(E) Effect of SEMA3A-G on cell collapse unadjusted for the amount of semaphorin secreted. Data are presented as mean ± SEM from at least 3 independent experiments; ^∗p < 0.05, ^∗∗p < 0.01 and ^∗∗∗p < 0.001.