Figure S3.

Generation and Characterization of Semaphorin-Neuropilin-Plexin Deletion Mutants in Zebrafish, Related to Figure 3

(A) Schematic illustrating the mutagenesis strategy to target sema3, plxna and nrp genes in zebrafish. Two-five sgRNAs were generated to mutagenize each zebrafish gene. Only sgRNAs verified to induce mutagenesis were injected into one-cell stage zebrafish embryos. Zebrafish were raised to ∼30 days post fertilization and fish length (mm), weight (mg) and % body fat were quantified.

(B) Results on fish length (mm), weight (mg) and % body fat for all deletion mutants (summarized in Figure 3B).

(C) Microphotographs and quantification of the density of α-melanocyte-stimulating hormone (αMSH) (red) and agouti-related peptide (AgRP) (green) immunoreactive (IR) fibers innervating the preoptic area (POA), anterior tuberal nucleus of hypothalamus (ATN), and lateral hypothalamic nucleus (LH) of 35-day-old wild-type zebrafish overexpressing NRP1 and NRP2; ^∗p < 0.05 in one-sample t tests.