REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Flag	Sigma-Aldrich	Cat# F3040; RRID: AB_439712
Glu-Glu	Covance	Cat# PEP-115P; RRID: AB_291283
Anti-Mouse IgG-HRP	Bio Rad	Cat #1721011; RRID: AB_11125936
Anti-rabbit IgG-HRP	Dako	Cat# P0448; RRID: AB_2617138
Biotin anti-human IgG	Sigma-Aldrich	Cat# SAB3701279 ; RRID: AB_AB_258559
Beta-actin	Cell Signaling Technology	Cat #4970; RRID: AB_2223172
Calnexin	Cell Signaling Technology	Cat #2679; RRID: AB_2228381
Anti-mouse AF488	Invitrogen	Cat#A28175; RRID: AB_2536161
Anti-rabbit AF568	Invitrogen	Cat#A10042; RRID: AB_2534017
Human Neuropilin 1	R&D Systems	Cat#AF3870; RRID: AB_884367
Human Neuropilin 2	R&D Systems	Cat#MAB2215; RRID: AB_2155370
betaIII tubulin	Covance	Cat#MRB-435P; RRID: AB_663339
Neuropeptide Y	Chemicon	Cat#AB1583; RRID: AB_2236176
α-MSH	Millipore	Cat#AB5087; RRID: AB_91683
Digoxigenin	Roche	Cat# 11093274910; RRID: AB_514497
Perilipin A	Sigma-Aldrich	Cat#P1873-200UL; RRID: AB_532267
Bacterial and Virus Strains
Subcloning Efficiency DH5α Competent Cells	Thermo Fisher Scientific	Cat#18265017
XL10-Gold	Agilent	Cat# 200315
AAV5-CMV.HI.eGFP-Cre.WPRE.SV40	This paper	NA
Biological Samples
Hypothalamus (human fetus at 14 weeks)	Dr Prevot, Inserm U1172, Lille, France	NA
Hypothalamus (human, 19 years ± 1.5 years) n = 2	Dr Prevot, Inserm U1172, Lille, France	NA
Chemicals, Peptides, and Recombinant Proteins
Lipofectamine 2000	Invitrogen	Cat#11668027
Semaphorin 3C	R&D Systems	Cat#5570-S3-050
Wheat Germ Agglutinin, Alexa Fluor 647 Conjugate	Invitrogen	Cat# W32466
Critical Commercial Assays
T7 Endonuclease I (T7E1)	New England Biolabs	Cat#M0302
QuantaBlu Fluorogenic Peroxidase Substrate Kit	Thermo Fisher Scientific	Cat#15169
PicoPure RNA Isolation Kit	Thermo Fisher Scientific	Cat# KIT0214
RNeasy Lipid Tissue Mini Kit	QIAGEN	Cat#74804
High-Capacity cDNA Reverse Transcription Kit	Thermo Fisher Scientific	Cat#4368814
Experimental Models: Cell Lines
HEK293	ATCC	CRL-1573
COS-7	ATCC	CRL-1651
U87MG	Gift from Gera Neufeld	NA
HUVEC	Gift from Gera Neufeld	NA
Experimental Models: Organisms/Strains
Zebrafish: WIK		ZDB-GENO-010531-2
Mouse (C57BL/6): Pomc-Cre	Balthasar et al., 2004	NA
Mouse (C57BL/6): ROSA-TdTomato reporter line	Madisen et al., 2010	NA
Mouse (C57BL/6): Pomc-Cre; Nrp2 loxP/loxP	This Paper	NA
Mouse (C57BL/6): Nrp2 loxP/loxP	Walz et al., 2002	NA
Recombinant DNA
Human Sema3A expression plasmid	OriGene	CAT#: RC213681
Human Sema3B expression plasmid	OriGene	CAT#: RC223532
Human Sema3C expression plasmid	OriGene	CAT#: RC205269
Human Sema3D expression plasmid	OriGene	CAT#: RC216032
Human Sema3E expression plasmid	OriGene	CAT#: RC216038
Human Sema3F expression plasmid	OriGene	CAT#: RC208333
Human Sema3G expression plasmid	OriGene	CAT#: RC222035
Human PlexinA1 expression plasmid	OriGene	CAT#: RC222057
Human PlexinA2 expression plasmid	OriGene	CAT#: RC221024
Human PlexinA3 expression plasmid	OriGene	CAT#: RC212456
Human PlexinA4 expression plasmid	OriGene	CAT#: RC226436
Human Neuropilin 1 expression plasmid	OriGene	CAT#: RC217035
Human Neuropilin 2 expression plasmid	OriGene	CAT#: RC220920
Software and Algorithms
Ensembl GeneTree	https://uswest.ensembl.org/Help/View?id=137	ENSGT00760000119048
Clustal Omega	https://www.ebi.ac.uk/Tools/msa/clustalo/	NA
Unrooted	http://pbil.univ-lyon1.fr/software/unrooted.html	NA
DanRer 10	Varshney et al., 2015	NA
TIDE	https://tide.nki.nl/	NA
VerifyBamID (v1.0)	http://csg.sph.umich.edu/kang/verifyBamID/	NA
EIGENSTRAT v4.2	Price et al., 2006	NA
PLINK v1.07	https://www.cog-genomics.org/plink2	NA
Fiji	https://fiji.sc/	NA
Prism	https://www.graphpad.com/scientific-software/prism/	NA
Modeler	https://salilab.org/modeller/	NA
Pymol	https://pymol.org/2/	NA