Skip to main content
. 2019 Feb 4;29(3):484–491.e6. doi: 10.1016/j.cub.2018.12.027

Figure 2.

Figure 2

Drosophila Snx27 Interacts with the PDZ Binding Motifs Present in the C Termini of Fmi and Stbm

(A) Alignment of the C-terminal PDZ binding motifs of Drosophila Fmi and Stbm with the optimized sequence for PDZ binding motif recognition by the PDZ domain of SNX27.

(B–E) GFP-nanotrap immunoprecipitations of HEK293T cells transiently transfected with constructs encoding for GFP-tagged tail regions of Fmi and Stbm (GFP-Fmi wild-type [WT], B and D, and GFP-Stbm WT, C and E) or the corresponding constructs deleted for the last three amino acids of the PDZ binding motif (GFP-FmiΔPDZbm, B and D, and GFP-StbmΔPDZbm, C and E).

(B and C) Samples immunoblotted for the presence of endogenous human SNX27.

(D and E) Cells co-transfected with mCherry-tagged Drosophila Snx27 and samples immunoblotted with anti-mCherry. Data are representative of similar data derived from three independent biological replicates.